With our peptide receptive easYmers® you can build a MHC class I repository relevant to your project and start producing pMHC complexes in your own lab. There are more than 50 HLA allotypes to choose from.
All MHC class I easYmer® molecules are biotinylated allowing you to make tetramers with your preferred labels. Stored frozen, MHC class I easYmers® are stable and peptide receptive for 24 months; once complexed with peptides, the resulting monomers are stable for years in the freezer.
We recommend that you use predictors (e.g. NetMHC series or NetMHCpan) as an aid to design your monomers. If the specific peptide-MHC class I combination of interest is not predicted to bind with a sufficiently low %Rank, there’s a high risk that the peptide will not support the formation of stable complexes. Thus, to ensure proper peptide loading and optimal staining of your CD8+ T cells, we strongly recommend that you experimentally verify successful peptide-MHC (pMHC) complex formation. To this end, the easYmer kit includes a simple FACS based protocol allowing you to validate proper peptide loading (click here for protocol).
The peptide loaded MHC monomers can be long-term stored frozen for later tetramerisation. When needed, these monomers can easily be tetramerised with labelled streptavidin (e.g. fluorophore label for analysis in a classical flow cytometry assay or metal-labels for analysis by CyTOF). For optimal HLA tetramer staining of CD8+ T cells, please follow our MHC class I tetramer staining protocol.
The easYmers® technology is highly flexibility and suitable for:
Evaluation of peptide-MHC class I interactions.
Epitope / Neo-epitope screening – small-scale tetramer production for screening of a large number of different epitopes in parallel.
Donor screening – large-scale tetramer production for screening of a single epitope in a large number of (donor) samples.
50+ HLA-A | HLA-B | HLA-C allotypes available
Mouse H2-Db, H2-Kb avilable
Add peptide to easYmer® and incubate. Add streptavidin-conjugate and stain T cells.