To ensure optimal staining of your CD8+ T cells, we strongly recommend to experimentally determine if the peptide of interest supports peptide MHC (pMHC) complex formation The easYmer kit includes a simple FACS based protocol allowing you to validate proper pMHC class I interaction (click here for protocol).
For optimal HLA tetramer staining of CD8+ T cells, please follow our MHC class I tetramer staining protocol.
The resulting HLA monomers can easily be tetramerized with labelled streptavidin (e.g. fluorophore label for analysis in a classical flow cytometry assay or metal-label for analysis by CyTOF). The pMHC monomers can also be long-term stored frozen for later tetramerization.
The easYmer technology is highly flexibility and suitable for:
- Evaluation of peptide-MHC class I interaction.
- Epitope / Neo-epitope screening – small-scale tetramer production for screening of a large number of different epitopes in parallel (i.e. for staining of up to five T cell samples per HLA tetramer).
- Donor screening – full-scale tetramer production for screening of a single epitope in a large number of samples (i.e. for staining of up to 50 T cell samples per HLA tetramer).
- 30+ HLA-A | HLA-B | HLA-C allotypes available including HLAs A*01 | A*02 | A*03
HLA-A*01:01, HLA-A*02:01, HLA-A*02:03, HLA-A*03:01, HLA-A*11:01, HLA-A*24:02, HLA-A*24:07, HLA-A*25:01, HLA-A*29:02, HLA-A*30:02, HLA-A*36:01, HLA-A*68:01, HLA-A*68:02
HLA-B*07:02, HLA-B*08:01, HLA-B*14:01, HLA-B*15:01, HLA-B*15:02, HLA-B*15:09, HLA-B*18:01, HLA-B*35:08, HLA-B*39:06, HLA-B*41:01, HLA-B*44:02, HLA-B*46:01, HLA-B*55:01, HLA-B*57:02
HLA-C*03:04, HLA-C*05:01, HLA-C*06:02, HLA-C*07:02